Unc93b Antibody

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ProSci
Product Code: PSI-4553
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4553-0.02mg0.02mg£150.00
Quantity:
PSI-4553-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Unc93b in human heart tissue lysate with Unc93b antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of Unc93b in human heart with Unc93b antibody at 2.5 μg/mL.
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Immunofluorescence of Unc93b in Human Heart cells with Unc93b antibody at 20 μg/mL.
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Western blot analysis of Unc93b in human heart tissue lysate with Unc93b antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of Unc93b in human heart with Unc93b antibody at 2.5 μg/mL.
Immunofluorescence of Unc93b in Human Heart cells with Unc93b antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Unc93b Antibody: IIAE1, UNC93, UNC93B, Unc-93B1, Protein unc-93 homolog B1
Application Note:
Unc93b antibody can be used for the detection of Unc93b by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Unc93b Antibody: The endoplasmic reticulum (ER) protein Unc93b, a human homolog of the C. elegans Unc93 gene, was initially identified by a forward genetic screen using N-ethyl-N-nitrosourea where a histidine-to-arginine substitution in Unc93b caused defects in Toll-like receptor (TLR) 3, 7 and 9 signaling. Unlike Unc93a, another homolog of the C. elegans Unc93 gene whose function is unknown, Unc93b specifically interacts with TLR3, 7 and 9; the histidine-to-arginine point mutation used to identify Unc93b abolishes this interaction. Mice carrying this point mutation are highly susceptible to infection with a number of viruses, indicating that Unc93b plays an important role in innate immunity.
Background References:
  • Tabeta K, Hoebe K, Janssen EM, et al. The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7, and 9. Nat. Immunol. 2006; 7:156-64.
  • Liu Y, Dodds P, Emilion G, et al. The human homolog of unc-93 maps to chromosome 6q27 - characterisation and analysis in sporadic epithelial ovarian cancer. BioMed Central Genetics2002; 3:20.
  • Brinkmann MM, Spooner E, Hoebe K, et al. The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling. J. Cell Biol. 2007; 177:265-75.
Buffer:
Unc93b Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Unc93b antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Unc93b.

The immunogen is located within amino acids 80 - 130 of Unc93b.
NCBI Gene ID #:
81622
NCBI Official Name:
unc-93 homolog B1 (C. elegans)
NCBI Official Symbol:
UNC93B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56, 66 kDa
Protein Accession #:
AAH33623
Protein GI Number:
116284350
Purification:
Unc93b Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
Multiple isoforms of Unc93b are known to exist. This antibody will not cross-react with Unc93a.
Swissprot #:
Q9H1C4
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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