CAPS1 Antibody

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ProSci
Product Code: PSI-4559
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4559-0.02mg0.02mg£150.00
Quantity:
PSI-4559-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of CAPS1 in human brain with CAPS1 antibody at 5 μg/mL.
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Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 μg/mL.
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Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of CAPS1 in human brain with CAPS1 antibody at 5 μg/mL.
Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
CAPS1 Antibody: CAPS, CAPS1, CADPS1, CAPS, KIAA1121, Calcium-dependent secretion activator 1, Calcium-dependent activator protein for secretion 1, CAPS-1
Application Note:
CAPS1 antibody can be used for detection of CAPS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CAPS1 Antibody: CAPS1 and its related protein CAPS2 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficienct neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. CAPS1 acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4, 5-bisphosphate and is thought to be a specific regulator of large dense-core vesicle fusion.
Background References:
  • Walent JH, Porter BW, and Martin TF. A novel 145 kD brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. Cell1992; 70:765-775.
  • Juckusch WJ, Speidel D, Sigler A, et al. CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins. Cell2007; 131:796-808.
  • Rupnick M, Kreft M, Sikdat SK, et al. Rapid regulated dense-core vesicle exocytosis requires the CAPS protein. Proc. Natl. Acad. Sci. USA2000; 97:5627-32.
Buffer:
CAPS1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CAPS1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human CAPS1.

The immunogen is located within the last 50 amino acids of CAPS1.
NCBI Gene ID #:
8618
NCBI Official Name:
Ca++-dependent secretion activator
NCBI Official Symbol:
CADPS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_899631
Protein GI Number:
34452713
Purification:
CAPS1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
Numerous isoforms of CAPS1 are known to exist; the lower molecular weight bands seen in the immunoblot image are likely to be these isoforms. This CAPS1 antibody is predicted to be specific to CAPS1 and not recognize CAPS2.
Swissprot #:
Q9ULU8
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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