CAPS1 Antibody

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ProSci
Product Code: PSI-4561
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4561-0.02mg0.02mg£150.00
Quantity:
PSI-4561-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.25 and (B) 0.5 μg/mL.
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Immunohistochemistry of CAPS1 in human brain with CAPS1 antibody at 5 μg/mL.
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Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 μg/mL.
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Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Immunohistochemistry of CAPS1 in human brain with CAPS1 antibody at 5 μg/mL.
Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
CAPS1 Antibody: CAPS, CAPS1, CADPS1, CAPS, KIAA1121, Calcium-dependent secretion activator 1, Calcium-dependent activator protein for secretion 1, CAPS-1
Application Note:
CAPS1 antibody can be used for detection of CAPS1 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CAPS1 Antibody: CAPS1 and its related protein CAPS2 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficienct neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. CAPS1 acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4, 5-bisphosphate and is thought to be a specific regulator of large dense-core vesicle fusion.
Background References:
  • Walent JH, Porter BW, and Martin TF. A novel 145 kD brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. Cell1992; 70:765-775.
  • Juckusch WJ, Speidel D, Sigler A, et al. CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins. Cell2007; 131:796-808.
  • Rupnick M, Kreft M, Sikdat SK, et al. Rapid regulated dense-core vesicle exocytosis requires the CAPS protein. Proc. Natl. Acad. Sci. USA2000; 97:5627-32.
Buffer:
CAPS1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen:
CAPS1 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of the human CAPS1.

The immunogen is located within amino acids 90 - 140 of CAPS1.
NCBI Gene ID #:
8618
NCBI Official Name:
Ca++-dependent secretion activator
NCBI Official Symbol:
CADPS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_899631
Protein GI Number:
34452713
Purification:
CAPS1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
Numerous isoforms of CAPS1 are known to exist. This CAPS1 antibody is predicted to be specific to CAPS1 and not recognize CAPS2.
Swissprot #:
Q9ULU8
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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