Syntaphilin Antibody
| Code | Size | Price |
|---|
| PSI-4635-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4635-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Syntaphilin Antibody: bA314N13.5, KIAA0374, Syntaphilin
Application Note:
Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.
Background References:
- Lao G, Scheuss V, Gerwin CM, et al. Syntaphilin: a syntaxin-1 clamp that controls SNARE assembly. Neuron2000; 25:191-201.
- Sorensen JB. SNARE complexes prepare for membrane fusion. Trends Neurosci.2005; 28:453-5.
- Das S, Gerwin C, and Sheng ZH. Syntaphilin binds to dynamin-1 and inhibits dynamin-dependent endocytosis. J. Biol. Chem.2003; 278:41221-6.
- Kang J-S, Tian J-H, Pan P-Y, et al. Docking of axonal mitochondria by syntaphilin controls their mobility and affects short-term facilitation. Cell2008; 132:137-148.
Buffer:
Syntaphilin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Syntaphilin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Syntaphilin.
The immunogen is located within amino acids 350 - 400 of Syntaphilin.
The immunogen is located within amino acids 350 - 400 of Syntaphilin.
NCBI Gene ID #:
9751
NCBI Official Name:
syntaphilin
NCBI Official Symbol:
SNPH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
O15079
Protein GI Number:
21362912
Purification:
Syntaphilin Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
O15079
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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