Wnt10b Antibody

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ProSci
Product Code: PSI-4619
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4619-0.02mg0.02mg£150.00
Quantity:
PSI-4619-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Wnt10b in human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 μg/mL.
2 / 2
Immunohistochemistry of Wnt10b in human skeletal muscle with Wnt10b antibody at 2.5 μg/mL.
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Western blot analysis of Wnt10b in human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 μg/mL.
Immunohistochemistry of Wnt10b in human skeletal muscle with Wnt10b antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
Wnt10b Antibody: SHFM6, WNT-12, WNT12, Protein Wnt-10b, Protein Wnt-12
Application Note:
Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
Wnt10b Antibody: Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.
Background References:
  • Lee FS, Lane TF, Kuo A, et al. Insertional mutagenesis identifies a member of the Wnt gene family as a candidate oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice. Proc. Natl. Acad. Sci. USA1995; 92:2268-72.
  • Nusse R and Varmus RE. Wnt genes. Cell1992; 69:1073-87.
  • Bui TD, Rankin J, Smith K, et al. A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas. Oncogene1997; 14:1249-53.
  • Bennett CN, Hodge CL, MacDougald OA, et al. Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells. Biochem. Biophys. Res. Commun.2003; 302:12-16.
Buffer:
Wnt10b Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b.

The immunogen is located within amino acids 130 - 180 of Wnt10b.
NCBI Gene ID #:
7480
NCBI Official Name:
wingless-type MMTV integration site family, member 10B
NCBI Official Symbol:
WNT10B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 43 kDa

Observed: 47 kDa
Protein Accession #:
NP_003385
Protein GI Number:
16936522
Purification:
Wnt10b Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.
Swissprot #:
O00744
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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