SATB1 Antibody
| Code | Size | Price |
|---|
| PSI-4679-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4679-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SATB1 Antibody: DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1
Application Note:
SATB1 antibody can be used for detection of SATB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SATB1 Antibody: Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a 'cage-like' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a 'genome organizer' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.
Background References:
- Dickinson LA and Kohwi-Shigematsu T. Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol.1995; 15:456-65.
- Szemes M, Gyorgy A, Pawaletz C, et al. Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain. Neurochem. Res.2006; 31:237-46.
- Purbey PK, Singh S, Kumar PP, et al. PDZ domain-mediated dimerization and homeodomain-directed specificity are required for high-affinity DNA binding by SATB1. Nucleic Acids Res.2008; 36:2107-22.
- Cai S, Han HJ and Kohwi-Shigematsu T. Tissue-specific nuclear architecture and gene expression regulated by SATB1. Nat. Genet.2003; 34:42-50.
Buffer:
SATB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SATB1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human SATB1.
The immunogen is located within the first 50 amino acids of SATB1.
The immunogen is located within the first 50 amino acids of SATB1.
NCBI Gene ID #:
6304
NCBI Official Name:
SATB homeobox 1
NCBI Official Symbol:
SATB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q01826
Protein GI Number:
417747
Purification:
SATB1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q01826
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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