Slc35D1 Antibody

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ProSci
Product Code: PSI-4607
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4607-0.02mg0.02mg£150.00
Quantity:
PSI-4607-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Slc35D1 in Daudi lysate with Slc35D1 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunocytochemistry of Slc35D1 in Daudi cells with Slc35D1 antibody at 5 μg/mL.
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Western blot analysis of Slc35D1 in Daudi lysate with Slc35D1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of Slc35D1 in Daudi cells with Slc35D1 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
Slc35D1 Antibody: UGTREL7, KIAA0260, UGTREL7, Solute carrier family 35 member D1, UGTrel7
Application Note:
Slc35D1 antibody can be used for detection of Slc35D1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
Slc35D1 Antibody: The solute carrier family Slc35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident Slc family member Slc35D1 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. These molecules can serve as substrates for chondroitin sulfate biosynthesis and mice lacking the Slc35D1 gene developed a lethal form of skeletal dysplasia with severe shortening of limbs and facial structures. Examination of epiphyseal cartilage in these mice revealed a decreased proliferating zone with round chrondrocytes, scarce matrices, and reduced proteoglycan aggregates. Loss of function mutations in human Slc35D1 cause Schneckenbecken dysplasia, a severe skeletal dysplasia.
Background References:
  • Ishida N and Kawakita M. Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35). Pflugers Arch.2004; 447:768-75.
  • Muraoka M, Kawakita M, and Ishita N. Molecular characterization of human UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, a novel nucleotide sugar transporter with dual substrate specificity. FEBS Lett.2001; 495:87-93.
  • Hiraoka S, Furuichi T, Nishimura G, et al. Nucleotide-sugar transporter Slc35D1 is critical to chondroitin sulfate synthesis in cartilage and skeletal development in mouse and human. Nat. Med.2007; 13:1363-7.
Buffer:
Slc35D1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (86%)
Immunogen:
Slc35D1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Slc35D1.

The immunogen is located within the last 50 amino acids of Slc35D1.
NCBI Gene ID #:
23169
NCBI Official Name:
solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
NCBI Official Symbol:
SLC35D1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055954
Protein GI Number:
14028875
Purification:
Slc35D1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is predicted to not cross-react with the highly homologous Slc35D2.
Swissprot #:
Q9NTN3
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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