Nanos1 Antibody

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ProSci
Product Code: PSI-4685
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4685-0.02mg0.02mg£150.00
Quantity:
PSI-4685-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of Nanos1 in rat brain tissue lysate with Nanos1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of nanos1 in human brain tissue with nanos1 antibody at 20 μg/mL.
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Western blot analysis of Nanos1 in rat brain tissue lysate with Nanos1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of nanos1 in human brain tissue with nanos1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Nanos1 Antibody: NOS1, SPGF12, NOS1, Nanos homolog 1, NOS-1
Application Note:
Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Nanos1 Antibody: Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.
Background References:
  • Jaruleska J, Kotecki M, Kusz K, et al. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev. Genes Evol.2003; 213:120-6.
  • Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science2003; 301:1239-41.
  • Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev.2003; 120:721-31.
  • Strumane K, Bonnomet A, Stove A, et al. E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion. Cancer Res.2006; 66:10007-15.
Buffer:
Nanos1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Nanos1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human Nanos1.

The immunogen is located within amino acids 160 - 210 of Nanos1.
NCBI Gene ID #:
340719
NCBI Official Name:
nanos homolog 1 (Drosophila)
NCBI Official Symbol:
NANOS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q8WY41
Protein GI Number:
41688589
Purification:
Nanos1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q8WY41
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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