MED4 Antibody
| Code | Size | Price |
|---|
| PSI-4729-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4729-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
MED4 Antibody: ARC36, VDRIP, DRIP36, TRAP36, HSPC126, ARC36, Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component
Application Note:
MED4 antibody can be used for detection of MED4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
MED4 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment.
Background References:
- Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.
- Gustafsson CM, Myers LC, Beve J, et al. Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J. Biol. Chem.1998; 273:30851-4.
- Rachez C, Lemon BD, Suldan Z, et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature1999; 398:824-8.
- Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.
Buffer:
MED4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen:
MED4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4.
The immunogen is located within the last 50 amino acids of MED4.
The immunogen is located within the last 50 amino acids of MED4.
NCBI Gene ID #:
29079
NCBI Official Name:
mediator complex subunit 4
NCBI Official Symbol:
MED4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_054885
Protein GI Number:
7661788
Purification:
MED4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
SPECIFICITY:
At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
Swissprot #:
Q9NPJ6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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