ASAH2 Antibody

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ProSci
Product Code: PSI-4743
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4743-0.02mg0.02mg£150.00
Quantity:
PSI-4743-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
ASAH2 Antibody: HNAC1, BCDase, LCDase, NCDase, N-CDase, HNAC1, Neutral ceramidase, Acylsphingosine deacylase 2
Application Note:
ASAH2 antibody can be used for detection of ASAH2 by Western blot at 1 and 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.
Background References:
  • Nilsson A and Duan RD. Alkaline sphingomyelinases and ceramidases of the gastrointestinal tract. Chem. Phys. Lipids1999; 102:97-105.
  • Tani M, Okino N, Mitsutake S, et al. Molecular Cloning of the Full-length cDNA Encoding Mouse Neutral Ceramidase. J. Biol. Chem.2000; 275:11229-34.
  • Tani M, Iida H, and Ito M. O-Glycosylation of Mucin-like Domain Retains the Neutral Ceramidase on the Plasma Membranes as a Type II Integral Membrane Protein. J. Biol. Chem.2003; 278:10523-30.
  • Osawa Y, Uchinami H, Bielawski J, et al. Roles for C16-ceramide and sphingosine 1-phosphate in regulating hepatocyte apoptosis in response to tumor necrosis factor-alpha. J. Biol. Chem.2005; 280:27879-87.
Buffer:
ASAH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2.

The immunogen is located within the last 50 amino acids of ASAH2.
NCBI Gene ID #:
56624
NCBI Official Name:
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
NCBI Official Symbol:
ASAH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_063946
Protein GI Number:
221218981
Purification:
ASAH2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q9NR71
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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