NENF Antibody
| Code | Size | Price |
|---|
| PSI-4753-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4753-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NENF Antibody: CIR2, SPUF, SCIRP10, CIR2, Neudesin, Cell immortalization-related protein 2, SPUF protein
Application Note:
NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
NENF Antibody: NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
Background References:
- Kimura I, Yoshioka M, Konishi M, et al. Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity. J. Neurosci. Res.2005; 79:287-94.
Buffer:
NENF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF.
The immunogen is located within the last 50 amino acids of NENF.
The immunogen is located within the last 50 amino acids of NENF.
NCBI Gene ID #:
29937
NCBI Official Name:
neuron derived neurotrophic factor
NCBI Official Symbol:
NENF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_037481
Protein GI Number:
7019545
Purification:
NENF Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q9UMX5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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