POFUT1 Antibody
| Code | Size | Price |
|---|
| PSI-4765-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4765-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
POFUT1 Antibody: DDD2, FUT12, O-FUT, OFUCT1, O-Fuc-T, O-FucT-1, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1
Application Note:
POFUT1 antibody can be used for the detection of POFUT1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
POFUT1 Antibody: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.
Background References:
- Wang Y, Shao L, Shi S, et al. Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase. J. Biol. Chem.2001; 276:40338-45.
- Loriol C, Dupuy F, Rampal R, et al. Molecular evolution of protein O-fucosyltransferase genes and splice variants. Glycobiology2006; 16:736-47.
- Stahl M, Uemura K, Ge C, et al. Roles of POFUT1 and O-fucose in mammalian Notch signaling. J. Biol. Chem.2008; 283:13638-51.
Buffer:
POFUT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%)
Immunogen:
POFUT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1.
The immunogen is located within amino acids 90 - 140 of POFUT1.
The immunogen is located within amino acids 90 - 140 of POFUT1.
NCBI Gene ID #:
23509
NCBI Official Name:
protein O-fucosyltransferase 1
NCBI Official Symbol:
POFUT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056167
Protein GI Number:
27436889
Purification:
POFUT1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Swissprot #:
Q9H488
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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