GPVI Antibody
| Code | Size | Price |
|---|
| PSI-4769-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4769-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
GPVI Antibody: GPIV, GPVI, BDPLT11, Platelet glycoprotein VI, Glycoprotein 6
Application Note:
GPVI antibody can be used for detection of GPVI by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
GPVI Antibody: Glycoprotein VI (GP6) is a 58kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. It is uniquely expressed by cells of the megakaryocytic/platelet lineage, and is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. Glycoprotein VI plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. GPVI deficiency can result in bleeding disorders. Further study should reveal the extent of GPVI involvement in thrombotic disease and allow the development of alternative anti-thrombotic compounds.
Background References:
- Jarvis GE, Atkinson BT, Snell DC, et al. Distinct roles of GPVI and integrin alpha(2) beta(1) in platelet shape change and aggregation induced by different collagens. Br. J. Pharmacol. 2002; 137:107-17.
- Inoue O, Suzuki-Inoue K, Dean WL, et al. Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2. J. Cell Biol. 2003;160:769-80.
- Clemetson JM, Polgar J, Magnenat E, et al. The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcαR and the natural killer receptors. J Biol. Chem. 1999; 274:29019-24.
- Jandrot-Perrus M, Busfield S, Lagrue AH, et al. Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily. Blood 2000; 96:1798-807.
Buffer:
GPVI Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GPVI antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of isoform 2 of the human GPVI.
The immunogen is located within the last 50 amino acids of GPVI.
The immunogen is located within the last 50 amino acids of GPVI.
NCBI Gene ID #:
51206
NCBI Official Name:
glycoprotein VI (platelet)
NCBI Official Symbol:
GP6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 68 kDa
Observed: 67 kDa
Observed: 67 kDa
Protein Accession #:
NP_057447
Protein GI Number:
143770755
Purification:
GPVI Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least three GPVI isoforms are known to exist; this antibody will detect the two longest isoforms. Because of its extensive glycosylation, GPVI often migrates at a higher than expected molecular weight in SDS-PAGE.
Swissprot #:
Q9HCN6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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