IL-34 Antibody

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ProSci
Product Code: PSI-4779
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4779-0.02mg0.02mg£150.00
Quantity:
PSI-4779-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of 1 ng of recombinant human IL-34 with IL-34 antibody at 1 μg/ml.
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Western blot analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of 1 ng of recombinant human IL-34 with IL-34 antibody at 1 μg/ml.

Documents

Further Information

Additional Names:
IL-34 Antibody: IL-34, C16orf77, Interleukin-34, IL-34
Application Note:
IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 μg/mL. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
IL-34 Antibody: Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.
Background References:
  • Lin H, Lee E, Hestir K, et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science2008; 320:807-11.
Buffer:
IL-34 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
IL-34 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-34.

The immunogen is located within amino acids 30 - 80 of IL-34.
NCBI Gene ID #:
146433
NCBI Official Name:
interleukin 34
NCBI Official Symbol:
IL34
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26 kDa

Observed: 42-48 kDa
Protein Accession #:
ACD13474
Protein GI Number:
187610438
Purification:
IL-34 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q6ZMJ4
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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