StrepII-tag Antibody
| Code | Size | Price |
|---|
| PSI-4217-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4217-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
StrepII-tag Antibody:
Application Note:
StrepII-tag antibody can be used for the detection of StrepII by Western blot at 0.25 μg/mL.
Background:
StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
Background References:
- Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
- Schmidt TGM, Koepe J, Frank R, et al. Molecular interactions between the Strep-tag affinity peptide and its cognate target, streptavidin. J. Mol. Biol.1996; 255:753-6.
Buffer:
StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
StrepII-tag antibody was raised against the amino acid sequence "gggWSHPQFEK".
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 100 kDa
Observed: 95 kDa
Observed: 95 kDa
Purification:
StrepII-tag Antibody is affinity chromatography purified via peptide column.
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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