StrepII-tag Antibody

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ProSci
Product Code: PSI-4335
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4335-0.02mg0.02mg£150.00
Quantity:
PSI-4335-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br> Loading: 30 ng of StrepII-tagged recombinant protein per lane. Antibodies: StrepII-tag 4335, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL Lane 2: 0.5 μg/mL Lane 3: 1 μg/mL
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<strong>Figure 2 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br> Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein. Antibodies: StrepII-tag 4335 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 1 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br> Loading: 30 ng of StrepII-tagged recombinant protein per lane. Antibodies: StrepII-tag 4335, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL Lane 2: 0.5 μg/mL Lane 3: 1 μg/mL
<strong>Figure 2 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br> Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein. Antibodies: StrepII-tag 4335 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
StrepII-tag Antibody:
Application Note:
WB: 0.25-1 μg/mL

Antibody validated: StrepII-tag antibody can be used for the detection of StrepII-tagged proteins
Background:
StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
Background References:
  • Schmidt and SKerra. Protein Eng.1993;6:109-22.
  • Schmidt et al. J. Mol. Biol. 1996;255:753-6.
Buffer:
StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-StrepII-tag antibody (4335) StrepII-tag antibody was raised against the amino acid sequence " srWSHPQFEKsh".
Physical State:
Liquid
Purification:
StrepII-tag Antibody is affinity chromatography purified via peptide column.
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Recombinant Protein Test (Figure 1&2): Anti-StrepII-tag antibodies (4335) detected StrepII-tagged recombinant protein at different concentrations.

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