POLR3F Antibody
| Code | Size | Price |
|---|
| PSI-4727-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4727-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
POLR3F Antibody: RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F, RNA polymerase III subunit C6
Application Note:
POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
POLR3F Antibody: The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.
Background References:
- Hu P, Wu S, Sun Y, et al. Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. Mol. Cell Biol.2002; 22:8044-55.
- Haeusler RA and Engelke DR. Spatial organization of transcription by RNA polymerase III. Nucleic Acids Res.2006; 34:4826-36. Epub 2006 Sep 13.
- Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.
Buffer:
POLR3F Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
POLR3F antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F.
The immunogen is located within the first 50 amino acids of POLR3F.
The immunogen is located within the first 50 amino acids of POLR3F.
NCBI Gene ID #:
10621
NCBI Official Name:
polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
NCBI Official Symbol:
POLR3F
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
CAC11110
Protein GI Number:
10636565
Purification:
POLR3F Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
Swissprot #:
Q9H1D9
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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