TRIM30 Antibody
| Code | Size | Price |
|---|
| PSI-4747-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4747-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TRIM30 Antibody: Rpt1, Rpt-1, Trim30, Rpt1, Tripartite motif-containing protein 30A, Down regulatory protein of interleukin-2 receptor
Application Note:
TRIM30 antibody can be used for detection of TRIM30 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TRIM30 Antibody: TRIM30 belongs to a family of the tripartite motif (TRIM) proteins involved in the regulation of cell proliferation, differentiation, development, oncogenesis, apoptosis and antiviral responses. The TRIM protein family is an expanding family of RING ('really interesting new gene') proteins, also known as RBCC proteins as they contain an RBCC motif, which comprises a RING domain, one or two B-boxes and a predicted coiled-coil region. Studies have shown that some TRIM family members are critical to innate immunity; TRIM5, TRIM19 and TRIM25, for example, have been shown to restrict viral infection. A recent study shows that TRIM30 functions as a negative modulator of the TLR signaling pathway, by targeting TAB2 and TAB3, and contributes to the inhibition of TLR-mediated NF-κB activation. The importance of TRIM30 in the attenuation or termination of NF-κB activation suggests that targeting of TAB2 and TAB3 by TRIM30alpha may be a mechanism for modulating many types of immune responses.
Background References:
- Nisole S, Stoye JP, and Saib A. TRIM family proteins: retroviral restriction and antiviral defence. Nat. Rev. Microbiol.2005; 3:799-808.
- Sakuma R, Noser JA, Ohmine S, et al. Rhesus monkey TRIM5 alpha restricts HIV-1 production through rapid degradation of viral Gag polyproteins. Nat. Med.2007; 13:631-635.?
- Gack MU, Shin YC, Joo CH, et al. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature2007; 446:916-920.?
- Patarca R, Freeman GJ, Schwartz J, et al. rpt-1, an intracellular protein from helper/inducer T cells that regulates gene expression of interleukin 2 receptor and human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA1988; 85:2733-7.
Buffer:
TRIM30 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Human: (80%)
Immunogen:
TRIM30 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of the mouse TRIM30.
The immunogen is located within amino acids 110 - 160 of TRIM30.
The immunogen is located within amino acids 110 - 160 of TRIM30.
NCBI Gene ID #:
20128
NCBI Official Name:
tripartite motif-containing 30
NCBI Official Symbol:
Trim30
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 107 kDa
Observed: 105 kDa
Observed: 105 kDa
Protein Accession #:
NP_033125
Protein GI Number:
125347379
Purification:
TRIM30 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
P15533
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier | TRIM30 Peptide | PSI-4747P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|