Integrin alpha 4 Antibody

Product Code: PSI-4827

Supplier: ProSci

Code	Size	Price

PSI-4827-0.02mg

0.02mg

£150.00

Quantity:

PSI-4827-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Western Blot (WB)

Images

1 / 2

Western blot analysis of Integrin alpha 4 using (A) 25 and (B) 100 ng of recombinant Integrin alpha 4 with Integrin alpha 4 antibody at 1 μg/mL.

2 / 2

Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 μg/mL.

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Documents

Further Information

Additional Names:

Integrin alpha 4 Antibody: IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D

Application Note:

Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Antibody can also be used for immunoflourescence starting at 2 μg/mL.

Antibody validated: Immunofluorescence in human samples. All other applications and species not yet tested.

Background:

Integrin alpha 4 Antibody: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta1, the gut mucosal homing receptor for peripheral T cells.

Background References:

Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol. 2008; 25:376-87.
Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29). Biochem J. 1995; 305:945-51.
Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 1993; 74:185-95.
Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem. 1991; 266:10241-5.

Buffer:

Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Integrin alpha 4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human Integrin alpha 4.

The immunogen is located within amino acids 150 - 200 of Integrin alpha 4.

NCBI Gene ID #:

3676

NCBI Official Name:

integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)

NCBI Official Symbol:

ITGA4

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Predicted: 17 kDa (recombinant protein)

Observed: 21 kDa

Protein Accession #:

NP_000876

Protein GI Number:

67191027

Purification:

Integrin alpha 4 Antibody is affinity chromatography purified via peptide column.

Research Area:

Infectious Disease

SPECIFICITY:

At least two isoforms of integrin alpha 4 are known to exist.

Swissprot #:

P13612

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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