CNOT4 Antibody
| Code | Size | Price |
|---|
| PSI-4813-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4813-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
CNOT4 Antibody: NOT4, NOT4H, CLONE243, NOT4, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4
Application Note:
CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
CNOT4 Antibody: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.
Background References:
- Albert TK, Hanzawa H, Legtenberg IA, et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J.2002; 21:355-64.
- Winkler GS, Albert TK, Dominguez C, et al. An altered specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. J. Mol. Biol.2004; 337:157-65.
- Mulder KW, Inagaki A, Cameroni E, et al. Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics2007; 176:181-92.
Buffer:
CNOT4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CNOT4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CNOT4.
The immunogen is located within amino acids 70 - 120 of CNOT4.
The immunogen is located within amino acids 70 - 120 of CNOT4.
NCBI Gene ID #:
4850
NCBI Official Name:
CCR4-NOT transcription complex, subunit 4
NCBI Official Symbol:
CNOT4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_037448
Protein GI Number:
56550057
Purification:
CNOT4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O95628
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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