ZBRK1 Antibody
| Code | Size | Price |
|---|
| PSI-4817-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4817-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ZBRK1 Antibody: ZFQR, ZBRK1, Zinc finger protein 350, KRAB zinc finger protein ZFQR
Application Note:
ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
ZBRK1 Antibody: ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.
Background References:
- Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell2000; 6:757-68.
- Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol.2001; 21:3609-15.
- Tan W, Kim S, and Boyer TG. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated Box-Zinc finger protein ZBRK1. J. Biol. Chem.2004; 279:55153-60.
Buffer:
ZBRK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZBRK1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human ZBRK1.
The immunogen is located within amino acids 410 - 460 of ZBRK1.
The immunogen is located within amino acids 410 - 460 of ZBRK1.
NCBI Gene ID #:
59348
NCBI Official Name:
zinc finger protein 350
NCBI Official Symbol:
ZNF350
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_067645
Protein GI Number:
59710107
Purification:
ZBRK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
Swissprot #:
Q9GZX5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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