CUEDC1 Antibody

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ProSci
Product Code: PSI-4829
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4829-0.02mg0.02mg£150.00
Quantity:
PSI-4829-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of CUEDC1 in rat liver tissue lysate with CUEDC1 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of CUEDC1 in human liver tissue with CUEDC1 antibody at 2 μg/mL.
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Immunofluorescence of CUEDC1 in human liver tissue with CUEDC1 antibody at 20 μg/mL.
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Western blot analysis of CUEDC1 in rat liver tissue lysate with CUEDC1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of CUEDC1 in human liver tissue with CUEDC1 antibody at 2 μg/mL.
Immunofluorescence of CUEDC1 in human liver tissue with CUEDC1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
CUEDC1 Antibody: CUE domain-containing protein 1
Application Note:
CUEDC1 antibody can be used for detection of CUEDC1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CUEDC1 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC1 (CUE domain-containing protein 1) is a 386 amino acid protein that contains one CUE domain, suggesting a possible role in protein trafficking and degradation pathways. Two isoforms of CUEDC1 exist due to alternative splicing events. Defects in the gene encoding CUEDC1 may be associated with early stage cervical cancer, implicating CUEDC1 as a potential tumor marker.
Background References:
  • Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J.2003; 22:1273-81.
  • Ponting CP. Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction. Biochem. J.2000; 351:527-35.
  • Prag G, Misra S, Jones EA, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell2003; 113:609-20.
  • Kang RS, Daniels CM, Francis SA, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell2003; 113:621-30.
Buffer:
CUEDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CUEDC1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CUEDC1.

The immunogen is located within amino acids 310 - 360 of CUEDC1.
NCBI Gene ID #:
404093
NCBI Official Name:
CUE domain containing 1
NCBI Official Symbol:
CUEDC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH56882
Protein GI Number:
34785153
Purification:
CUEDC1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
CUEDC1 antibody will not cross-react with CUEDC2.
Swissprot #:
Q9NWM3
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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