CUEDC2 Antibody

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ProSci
Product Code: PSI-4839
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4839-0.02mg0.02mg£150.00
Quantity:
PSI-4839-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 μg/mL.
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Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
CUEDC2 Antibody: C10orf66, bA18I14.5, C10orf66, HOYS6, CUE domain-containing protein 2
Application Note:
CUEDC2 antibody can be used for detection of CUEDC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
CUEDC2 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC2 (CUE domain-containing protein 2) was found through a yeast two-hybrid screening as a protein that interacts with the progesterone receptor (PR) and promotes progesterone-induced PR degradation by the ubiquitin-proteasome pathway. CUEDC2 also decreases the sumoylation of PR. CUEDC2 has also been found to interact with IKK-alpha and IKK-beta and decrease the activation of NF-κB by decreasing the activation of IKK.
Background References:
  • Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003; 22:1273-81.
  • Ponting CP. Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction. Biochem. J. 2000; 351:527-35.
  • Prag G, Misra S, Jones EA, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell 2003; 113:609-20.
  • Kang RS, Daniels CM, Francis SA, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell 2003; 113:621-30.
Buffer:
CUEDC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CUEDC2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2.

The immunogen is located within the last 50 amino acids of CUEDC2.
NCBI Gene ID #:
79004
NCBI Official Name:
CUE domain containing 2
NCBI Official Symbol:
CUEDC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 32 kDa

Observed: 34 kDa
Protein Accession #:
NP_076945
Protein GI Number:
148596996
Purification:
CUEDC2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
CUEDC2 antibody will not cross-react with CUEDC1.
Swissprot #:
Q9H467
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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