Dact3 Antibody
| Code | Size | Price |
|---|
| PSI-4871-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4871-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Dact3 Antibody: RRR1, DAPPER3, RRR1, Dapper homolog 3, Antagonist of beta-catenin Dapper homolog 3
Application Note:
Dact3 antibody can be used for detection of Dact3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Background:
Dact3 Antibody: The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact3, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact3 is expressed in the ventral region of maturing somites, limb bud and branchial arch mesenchyme, embryonic CNS, and the adult brain. Recent evidence shows that Dact3 acts as a negative regulator Wnt/beta-catenin signaling that is repressed at the transcriptional level in colorectal cancer and this repression is associated with bivalent histone modifications. This repression can be reversed by pharmacological agents that targets both histone methylation and deacetylation, suggesting that Dact3 may be a potential target for therapeutic treatment of this cancer. At least three isoforms of Dact3 are known to exist.
Background References:
- Shimigori T, VanSant J, Paik E, et al. Members of the Wnt, Fz, and Frp gene families expressed in postnatal mouse cerebral cortex. J. Comp. Neurol.2004; 473:496-510.
- Cheyette BNR, Waxman JS, Miller JR, et al. Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Dev. Cell2002; 2:449-61.
- Katoh M and Katoh M. Identification and characterization of human DAPPER1 and DAPPER2 genes in silico. Int. J. Oncol.22:907-13.
- Fisher DA, Kivimae S, Hoshino J, et al. Three Dact gene family members are expressed during embryonic development and in the adult brains of mice. Dev. Dyn.2006; 235:2620-30.
Buffer:
Dact3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Dact3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Dact3.
The immunogen is located within amino acids 370 - 420 of Dact3.
The immunogen is located within amino acids 370 - 420 of Dact3.
NCBI Gene ID #:
147906
NCBI Official Name:
dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis)
NCBI Official Symbol:
DACT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q96B18
Protein GI Number:
119368655
Purification:
Dact3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q96B18
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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