MARCH8 Antibody

Product Code: PSI-4885

Supplier: ProSci

Code	Size	Price

PSI-4885-0.02mg

0.02mg

£150.00

Quantity:

PSI-4885-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

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Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.

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Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 μg/mL.

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Documents

Further Information

Additional Names:

MARCH8 Antibody: MIR, c-MIR, RNF178, MARCH-VIII, MIR, E3 ubiquitin-protein ligase MARCH8, Cellular modulator of immune recognition

Application Note:

MARCH8 antibody can be used for detection of MARCH8 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Background:

MARCH8 Antibody: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Background References:

Coscoy L, Sanchez DJ, and Ganem D. A novel class of herpesvirus-encoded membrane-bound E3 ubiquitin ligases regulates endocytosis of proteins involved in immune recognition. J. Cell Biol. 2001; 155:126573.
Goto E, Ishido S, Sato Y, et al. c-MIR, a human E3 ubiquitin ligase, is a functional homolog of herpesvirus proteins MIR1 and MIR2 and has similar activity. J. Biol. Chem.2003; 278:14657-68.
Ohmura-Hoshino M, Goto E, Matsuki Y, et al. A novel family of membrane-bound E3 ubiquitin ligases. J. Biochem.2006; 140:147-54.
Bartee E, Mansouri M, Hovey Nerenberg BT, et al. Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins. J. Virol.2004; 78:1109-20.

Buffer:

MARCH8 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Bovine: (76%)

Immunogen:

MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8.

The immunogen is located within the last 50 amino acids of MARCH8.

NCBI Gene ID #:

220972

NCBI Official Name:

membrane-associated ring finger (C3HC4) 8

NCBI Official Symbol:

Mar-08

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_659458

Protein GI Number:

50539410

Purification:

MARCH8 Antibody is affinity chromatography purified via peptide column.

Research Area:

Innate Immunity

Swissprot #:

Q5T0T0

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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MARCH8 Peptide	PSI-4885P	ProSci	Summary Details