IL-17 Antibody

Contact us
ProSci
Product Code: PSI-4877
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4877-0.02mg0.02mg£150.00
Quantity:
PSI-4877-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

1 / 3
<strong>Figure 1 Western Blot Validation in Human THP-1 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-17 4877 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2 / 3
<strong>Figure 2 Western Blot Validation in A-20 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-17 4877 (A: 2 μg/mL and B: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
3 / 3
<strong>Figure 3 Recombinant Protein Test </strong><br> Loading: 30 ng of human IL-17 recombinant protein per lane. Antibodies: IL-17 4877, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.125 μg/mL Lane 2: 0.25 μg/mL Lane 3: 0.5 μg/mL
❮❯

<strong>Figure 1 Western Blot Validation in Human THP-1 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-17 4877 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in A-20 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-17 4877 (A: 2 μg/mL and B: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Recombinant Protein Test </strong><br> Loading: 30 ng of human IL-17 recombinant protein per lane. Antibodies: IL-17 4877, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.125 μg/mL Lane 2: 0.25 μg/mL Lane 3: 0.5 μg/mL

Documents

Further Information

Additional Names:
IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
Application Note:
WB: 0.125-4 μg/mL.

Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Background:
IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
Background References:
  • Miossec. Curr. Opin. Rheumatol.2000; 12:181-5.
  • Paunovic et al. Rheumatol.2008; 47:771-6.
  • Steinman. Nat. Med.2007; 13:139-145.
Buffer:
IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.

The immunogen is located within amino acids 40 - 90 of IL-17.
ISOFORMS:
Human IL-17 has only one isoform (155aa, 17.5kD). Mouse IL-17 has one isoform (158aa, 17.5kD) and rat IL-17 also has one isoform (150aa, 16.9kD). 4877 can detect only human isoform, but cannot detect mouse and rat.
NCBI Gene ID #:
3605
NCBI Official Name:
interleukin 17A
NCBI Official Symbol:
IL17A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 17.5kD

Observed: 20 kD (Post-modification: 1 N-linked glycosylation)
Protein Accession #:
NP_002181
Protein GI Number:
4504651
Purification:
IL-17 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q16552
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Recombinant Protein Test (Figure 3): Anit-IL-17 antibodies (4877) detected human IL-17 recombinant protein at different concentrations.

Related Products

Product NameProduct CodeSupplier 
IL-17 PeptidePSI-4877PProSci Summary Details