JPH1 Antibody

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ProSci
Product Code: PSI-4917
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4917-0.02mg0.02mg£150.00
Quantity:
PSI-4917-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 μg/mL.
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Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
JPH1 Antibody: JP1, JP-1, JP1, Junctophilin-1, Junctophilin type 1
Application Note:
JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
JPH1 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).
Background References:
  • Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell.2000; 6:11-22.
  • Kakizawa S, Kishimoto Y, Hashimoto K, et al. Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. EMBO J.2007; 26:1924-33.
  • Nishi M, Sakagami H, Komazaki S, et al. Coexpression of junctophilin type 3 and type 4 in brain. Brain Res. Mol. Brain Res.2003; 118:102-10.
  • Phimister AJ, Lango J, Lee EH, et al. Conformation-dependent stability of Junctophilin 1 (JP1) and Ryanodine Receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J. Biol. Chem.2007; 282:8867-77.
Buffer:
JPH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (87%)
Immunogen:
JPH1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human JPH1.

The immunogen is located within amino acids 470 - 520 of JPH1.
NCBI Gene ID #:
56704
NCBI Official Name:
junctophilin 1
NCBI Official Symbol:
JPH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAI39833
Protein GI Number:
145337941
Purification:
JPH1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q9HDC5
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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