JPH2 Antibody

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ProSci
Product Code: PSI-4929
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4929-0.02mg0.02mg£150.00
Quantity:
PSI-4929-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of JPH2 in mouse brain tissue lysate with JPH2 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunohistochemistry of JPH2 in mouse skeletal muscle tissue with JPH2 antibody at 2.5 μg/mL.
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Western blot analysis of JPH2 in mouse brain tissue lysate with JPH2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of JPH2 in mouse skeletal muscle tissue with JPH2 antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
JPH2 Antibody: JP4, JPHL1, KIAA1831, Junctophilin-4, Junctophilin-like 1 protein, JP-4
Application Note:
JPH2 antibody can be used for detection of JPH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
JPH2 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH2-null mice died of embryonic cardiac arrest and human patients with mutations in the JPH2 gene showed hypertrophic cardiomyopathy, demonstrating the importance of this protein. Multiple isoforms of JPH2 are known to exist.
Background References:
  • Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell.2000; 6:11-22.
  • Kakizawa S, Kishimoto Y, Hashimoto K, et al. Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. EMBO J.2007; 26:1924-33.
  • Nishi M, Sakagami H, Komazaki S, et al. Coexpression of junctophilin type 3 and type 4 in brain. Brain Res. Mol. Brain Res.2003; 118:102-10.
  • Matsushita Y, Furukawa T, Kasanuki H, et al. Mutation of junctophilin type 2 associated with hypertrophic cardiomyopathy. J. Hum. Genet.2007; 52:543-8.
Buffer:
JPH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (88%)
Immunogen:
JPH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human JPH2.

The immunogen is located within amino acids 470 - 520 of JPH2.
NCBI Gene ID #:
84502
NCBI Official Name:
junctophilin 4
NCBI Official Symbol:
JPH4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065166
Protein GI Number:
21704281
Purification:
JPH2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q96JJ6
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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