Gle1 Antibody

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ProSci
Product Code: PSI-4963
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4963-0.02mg0.02mg£150.00
Quantity:
PSI-4963-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Gle1 in mouse brain tissue lysate with Gle1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of Gle1 in mouse brain tissue with Gle1 antibody at 2.5 μg/mL.
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Immunofluorescence of gle1 in mouse brain tissue with gle1 antibody at 5 μg/mL.
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Western blot analysis of Gle1 in mouse brain tissue lysate with Gle1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Gle1 in mouse brain tissue with Gle1 antibody at 2.5 μg/mL.
Immunofluorescence of gle1 in mouse brain tissue with gle1 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
Gle1 Antibody: LCCS, GLE1L, LCCS1, hGLE1, Nucleoporin GLE1, GLE1-like protein
Application Note:
Gle1 antibody can be used for detection of Gle1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Gle1 Antibody: The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.
Background References:
  • Murphy R and Wente SR. An RNA-export mediator with an essential nuclear export signal. Nature1996; 383:357-60.
  • Watkins JL, Murphy R, Emtage JL, et al. The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export. Proc. Natl. Acad. Sci. USA1998; 95:6779-84.
  • Kendirgi F, Rexer DJ, Alcazar-Roman AR, et al. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: A conserved mechanism in the export of Hsp70 mRNA. Mol. Biol. Cell2005; 16:4304-15.
  • Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell. Proteomics2004; 3:145-55.
Buffer:
Gle1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Gle1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Gle1.

The immunogen is located within amino acids 540 - 590 of Gle1.
NCBI Gene ID #:
2733
NCBI Official Name:
GLE1 RNA export mediator homolog (yeast)
NCBI Official Symbol:
GLE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001003722
Protein GI Number:
51317384
Purification:
Gle1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q53GS7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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