TREX1 Antibody
| Code | Size | Price |
|---|
| PSI-4969-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4969-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TREX1 Antibody: CRV, AGS1, DRN3, HERNS, Three prime repair exonuclease 1, DNase III
Application Note:
TREX1 antibody can be used for detection of TREX1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TREX1 Antibody: Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis.
Background References:
- Mazur DJ and Perrino FW. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3→5 exonucleases. J. Biol. Chem. 1999; 274:19655-60.
- Cortez D, Guntuku S, Qin J, et. al. ATR and ATRIP: partners in checkpoint signaling. Science 2001 294:1713-6
- Stetson DB, Ko JS, Heidmann T, et al. Trex1 prevents cell-intrinsic initiation of autoimmunity. Cell 2008 134:587-98.
- Mazur DJ and Perrino FW. Structure and expression of the TREX1 and TREX2 3'→5' exonuclease genes. J. Biol. Chem. 2001; 276:14718-27.
Buffer:
TREX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen:
TREX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1.
The immunogen is located within amino acids 220 - 270 of TREX1.
The immunogen is located within amino acids 220 - 270 of TREX1.
NCBI Gene ID #:
11277
NCBI Official Name:
three prime repair exonuclease 1
NCBI Official Symbol:
TREX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 33, 35, 41 kDa
Observed: 31 kDa
Observed: 31 kDa
Protein Accession #:
NP_057465
Protein GI Number:
7705353
Purification:
TREX1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
TREX1 antibody will not cross-react with the related protein TREX2. At least three isoforms of TREX1 are known to exist; this antibody will recognize all three isoforms.
Swissprot #:
Q9NSU2
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier | TREX1 Peptide | PSI-4969P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|