TREX2 Antibody

Contact us
ProSci
Product Code: PSI-4971
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4971-0.02mg0.02mg£150.00
Quantity:
PSI-4971-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of TREX2 in rat liver tissue lysate with TREX2 antibody at 2.5 μg/mL.
2 / 3
Immunohistochemistry of TREX2 in human liver tissue with TREX2 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of TREX2 in Human Liver cells with TREX2 antibody at 20 μg/mL.
❮❯

Western blot analysis of TREX2 in rat liver tissue lysate with TREX2 antibody at 2.5 μg/mL.
Immunohistochemistry of TREX2 in human liver tissue with TREX2 antibody at 2.5 μg/mL.
Immunofluorescence of TREX2 in Human Liver cells with TREX2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TREX2 Antibody: Three prime repair exonuclease 2
Application Note:
TREX2 antibody can be used for detection of TREX2 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TREX2 Antibody: TREX2 is one of two major human 3' to 5' exonucleases which are required for checkpoint signaling after DNA damage. While the related protein TREX1 is required for ATR expression and mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis, less is known about TREX2. Like TREX1, TREX2 is ubiquitously expressed in all tissues examined. X-ray crystallography studies of TREX2-single strand DNA complexes revealed that TREX2 binds DNA as a dimer and may act to displace the second DNA strand, suggesting that TREX2 unzips and denatures double stranded DNA and feed the substrate DNA strand into the TREX2 active site. At least two isoforms of TREX2 are known to exist.
Background References:
  • Mazur DJ and Perrino FW. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3→5 exonucleases. J. Biol. Chem.1999; 274:19655-60.
  • Cortez D, Guntuku S, Qin J, et. al. ATR and ATRIP: partners in checkpoint signaling. Science2001; 294:1713-6
  • Crow YJ, Hayward BE, Parmar R, et al. Mutations in the gene encoding the 3-5DNA exonuclease TREX1 cause Aicardi-Goutieres syndrome at the AGS1 locus. Nat. Genet. 2006; 38:917-20
  • Mazur DJ and Perrino FW. Structure and expression of the TREX1 and TREX2 3'→5' exonuclease genes. J. Biol. Chem.2001; 276:14718-27.
Buffer:
TREX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TREX2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TREX2.

The immunogen is located within the last 50 amino acids of TREX2.
NCBI Gene ID #:
11219
NCBI Official Name:
three prime repair exonuclease 2
NCBI Official Symbol:
TREX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9BQ50
Protein GI Number:
47606206
Purification:
TREX2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
This TREX2 antibody will not cross-react with the related protein TREX1.
Swissprot #:
Q9BQ50
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
TREX2 PeptidePSI-4971PProSci Summary Details