RNAse H2A Antibody
| Code | Size | Price |
|---|
| PSI-4979-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4979-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RNAse H2A Antibody: AGS4, JUNB, RNHL, RNHIA, RNASEHI, Ribonuclease H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H2 subunit A
Application Note:
RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RNAse H2A Antibody: Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.
Background References:
- Stein H and Hausen P. Enzyme from calf thymus degrading the RNA moiety of DNA-RNA hybrids: effect on DNA-dependent RNA polymerase. Science 1969; 166:393-5.
- Cerritelli SM and Crouch RJ. Ribonuclease H: the enzymes in eukaryotes. FEBS J. 2009; 276:1494-505.
- Jeong HS, Backlund PS, Chen HC, et al. RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. Nuc. Acids Res. 2004; 32:407-14.
- Crow YJ, Leitch A, Hayward BE, et al. Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nat. Genet. 2006; 38:910-6.
Buffer:
RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A.
The immunogen is located within amino acids 190 - 240 of RNAse H2A.
The immunogen is located within amino acids 190 - 240 of RNAse H2A.
NCBI Gene ID #:
10535
NCBI Official Name:
ribonuclease H2, subunit A
NCBI Official Symbol:
RNASEH2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 33 kDa
Observed: 33 kDa
Observed: 33 kDa
Protein Accession #:
O75792
Protein GI Number:
20981704
Purification:
RNAse H2A Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O75792
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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