Beta-actin Antibody
| Code | Size | Price |
|---|
| PSI-5009-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5009-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Beta-actin Antibody: BRWS1, PS1TP5BP1, Actin, cytoplasmic 1, Beta-actin
Application Note:
beta-actin antibody can be used for the detection of beta-actin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
Beta-actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Background References:
- Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol.2004; 36:1890-909.
- Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing Nat. Rev.2005; 6:583-9.
Buffer:
Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Pig: (100%), Horse: (100%), Chicken: (100%), Guinea pig: (100%), Bovine: (100%), Rabbit: (100%)
Immunogen:
Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human beta-actin.
The immunogen is located within amino acids 90 - 140 of Beta-actin.
The immunogen is located within amino acids 90 - 140 of Beta-actin.
NCBI Gene ID #:
60
NCBI Official Name:
actin, beta
NCBI Official Symbol:
ACTB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH02409
Protein GI Number:
12803203
Purification:
Beta-actin Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P60709
User NOte:
Optimal dilutions for each application to be determined by the researcher.