SPG11 Antibody

Product Code: PSI-5015

Supplier: ProSci

Code	Size	Price

PSI-5015-0.02mg

0.02mg

£150.00

Quantity:

PSI-5015-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

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Western blot analysis of SPG11 in mouse heart tissue lysate with SPG11 antibody at (A) 0.5 and (B) 1 μg/mL.

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Immunohistochemistry of SPG11 in mouse brain tissue with SPG11 antibody at 2.5 μg/mL.

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Documents

Further Information

Additional Names:

SPG11 Antibody: KIAA1840, KIAA1840, Spatacsin, Colorectal carcinoma-associated protein

Application Note:

SPG11 antibody can be used for detection of SPG11 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Background:

SPG11 Antibody: Hereditary spastic paraplegias (HSPs) are genetically and phenotypically heterogeneous disorders.? Spastic paraplegia with thinning of the corpus callosum (ARHSP-TCC) is a relatively frequent form of complicated hereditary spastic paraplegia (cHSP) in which mental retardation and muscle stiffness at onset are followed by slowly progressive paraparesis and cognitive deterioration. Mutations of the SPG11 gene encoding the spatacsin protein have been identified as a major cause of HSP-TCC. Spatacsin is a potential transmembrane protein that is phosphorylated upon DNA damage. It is expressed in all structures of the brain, with a high expression in the cerebellum. SPG11 mutations may occur more frequently in familial than sporadic forms of cHSP without TCC. Kjellin syndrome is found to be associated with mutations in not only the SPG15 gene but also SPG11 gene.? Recent studies show Parkinsonism may initiate SPG11-linked HSP TCC and that SPG11 may cause juvenile Parkinsonism.

Background References:

Stevanin G, Azzedine H, Denora P, et al. Mutations in SPG11, encoding spatacsin, are a major cause of spastic paraplegia with thin corpus callosum. Nature Gen.2007; 39:366-372.
Paisan-Ruiz C, Dogu O, Yilmaz A, et al. SPG11 mutations are common in familial cases of complicated hereditary spastic paraplegia. Neurology2008; 70:1384-9.?
Crimella C, Arnoldi A, Crippa F, et al. Point mutations and a large intragenic deletion in SPG11 in complicated spastic paraplegia without thin corpus callosum. J. Med. Genet.2009; 46:345-51.?
Orl?n H,?Melberg A, Raininko R, et al. SPG11 mutations cause Kjellin syndrome, a hereditary spastic paraplegia with thin corpus callosum and central retinal degeneration. Am. J. Med. Genet. B Neuropsychiatr. Genet.2009; epub.?

Buffer:

SPG11 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

SPG11 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SPG11.

The immunogen is located within amino acids 2360 - 2410 of SPG11.

NCBI Gene ID #:

80208

NCBI Official Name:

spastic paraplegia 11 (autosomal recessive)

NCBI Official Symbol:

SPG11

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

AAI53880

Protein GI Number:

158253417

Purification:

SPG11 Antibody is affinity chromatography purified via peptide column.

Research Area:

Apoptosis

Swissprot #:

Q96JI7

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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