GNPDA1 Antibody

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ProSci
Product Code: PSI-5049
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5049-0.02mg0.02mg£150.00
Quantity:
PSI-5049-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.
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Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.
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Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.
Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
GNPDA1 Antibody: GPI, HLN, GNP1, GNPI, GNPDA, KIAA0060, Glucosamine-6-phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, GNPDA 1
Application Note:
GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.
Background References:
  • Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9.
  • Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem.2003; 88:932-40.
  • Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett.2003; 551:63-70.
Buffer:
GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1.

The immunogen is located within the last 50 amino acids of GNPDA1.
NCBI Gene ID #:
10007
NCBI Official Name:
glucosamine-6-phosphate deaminase 1
NCBI Official Symbol:
GNPDA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAC05123
Protein GI Number:
2935438
Purification:
GNPDA1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
GNPDA1 is predicted to not cross-react with GNPDA2.
Swissprot #:
P46926
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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