PCDH12 Antibody

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ProSci
Product Code: PSI-5071
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5071-0.02mg0.02mg£150.00
Quantity:
PSI-5071-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunohistochemistry of PCDH12 in rat lung tissue with PCDH12 antibody at 5 μg/mL.
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Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PCDH12 in rat lung tissue with PCDH12 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
PCDH12 Antibody: VECAD2, VE-cadherin-2, Protocadherin-12, Vascular cadherin-2, VE-cad-2
Application Note:
PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
PCDH12 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.
Background References:
  • Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol.2002; 14:557-62.
  • Telo P, Breviaro F, Huber P, et al. Identification of a novel cadherin (vascular endothelial cadherin-2) located at intercellular junctions in endothelial cells. J. Biol. Chem.1998; 28:17565-72.
  • Rampon C, Prandini MH, Bouillot S, et al. Protocadherin 12 (VE-cadherin 2) is expressed in endothelial, trophoblast, and mesangial cells. Exp. Cell Res.2005; 302:48-60.
  • Rampon C, Bouillot S, Climescu-Haulica A, et al. Protocadherin 12 deficiency alters morphogenesis and transcriptional profile of the placenta. Physiol. Genomics2008; 34:193-204.
Buffer:
PCDH12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12.

The immunogen is located within amino acids 30 - 80 of PCDH12.
NCBI Gene ID #:
51294
NCBI Official Name:
protocadherin 12
NCBI Official Symbol:
PCDH12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 128 kDa

Observed: 125 kDa
Protein Accession #:
NP_057664
Protein GI Number:
7706113
Purification:
PCDH12 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is predicted to not cross-react with PCDH18.
Swissprot #:
Q9NPG4
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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