OTUD4 Antibody

ProSci
Product Code: PSI-5075
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5075-0.02mg0.02mg£150.00
Quantity:
PSI-5075-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μg/mL.
2 / 3
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 μg/mL.

Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 μg/mL.
Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 μg/mL.

Further Information

Additional Names:
OTUD4 Antibody: HIN1, DUBA6, HSHIN1, HIN1, KIAA1046, OTU domain-containing protein 4, HIV-1-induced protein HIN-1
Application Note:
OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).
Background References:
  • Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59.
Buffer:
OTUD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4.

The immunogen is located within the last 50 amino acids of OTUD4.
NCBI Gene ID #:
54726
NCBI Official Name:
OTU domain containing 4
NCBI Official Symbol:
OTUD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 115, 123 kDa

Observed: 125 kDa
Protein Accession #:
NP_001096123
Protein GI Number:
156630992
Purification:
OTUD4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family.
Swissprot #:
Q01804
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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