Bora Antibody
| Code | Size | Price |
|---|
| PSI-5117-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5117-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Bora Antibody: C13orf34, C13orf34, Protein aurora borealis, HsBora
Application Note:
Bora antibody can be used for detection of Bora by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
Background References:
- Berdnik D and Knoblich JA. Drosophila Aurora A is required for centrosome maturation and Actin-dependent asymmetric protein localization during mitosis. Curr. Biol.2002; 12:640-647.
- Wiese C and O'Brien LL. What's so Bor(a)ing about Aurora A activation Dev. Cell2006; 11:133-134.
- Hutterer A, Berdnik D, Wirtz-Peitz F, et al. Mitotic activation of the kinase Aurora A requires its binding partner Bora. Dev. Cell2006; 11:147-157.
- Fu J, Bian M, Jiang Q, et al.. Roles of Aurora kinases in mitosis and tumorigenesis. Mol. Cancer Res.2007; 5:1-10.
Buffer:
Bora Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bora antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human Bora.
The immunogen is located within amino acids 70 - 120 of Bora.
The immunogen is located within amino acids 70 - 120 of Bora.
NCBI Gene ID #:
79866
NCBI Official Name:
bora, aurora kinase A activator
NCBI Official Symbol:
BORA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q6PGQ7
Protein GI Number:
74737659
Purification:
Bora Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q6PGQ7
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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