APP Antibody

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ProSci
Product Code: PSI-5129
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5129-0.02mg0.02mg£150.00
Quantity:
PSI-5129-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of APP in mouse brain tissue lysate with APP antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of APP in mouse brain tissue with APP antibody at 2.5 μg/mL.
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Immunofluorescence of APP in mouse brain tissue with APP antibody at 20 μg/mL.
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Western blot analysis of APP in mouse brain tissue lysate with APP antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of APP in mouse brain tissue with APP antibody at 2.5 μg/mL.
Immunofluorescence of APP in mouse brain tissue with APP antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP
Application Note:
APP antibody can be used for detection of APP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. Recently, Death Receptor 6 (DR6) was found to interact with an amino-terminal fragment of the beta-amyloid protein (N-APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning during development, suggesting that these two proteins are involved in neural development and may possibly play a role in Alzheimer's disease.
Background References:
  • Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature1988; 331:525-77.
  • Selkoe DJ. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu Rev Cell Biol1994; 10:373-403.
  • Nikolaev A, McLaughlin T, O'Leary DD, et al. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature2009; 457:981-9.
Buffer:
APP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (94%), Guinea pig: (89%)
Immunogen:
APP antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APP.

The immunogen is located within amino acids 30 - 80 of APP.
NCBI Gene ID #:
351
NCBI Official Name:
amyloid beta (A4) precursor protein
NCBI Official Symbol:
APP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
CAA30050
Protein GI Number:
28721
Purification:
APP Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
P05067
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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