FTO Antibody
| Code | Size | Price |
|---|
| PSI-5137-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5137-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
FTO Antibody: ALKBH9, KIAA1752, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein
Application Note:
FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
FTO Antibody: Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
Background References:
- Scuteri A, Sanna S, Chen W-M, et al. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PloS Genet.2007; 3:e115.
- Gerken T, Girard CA, Tung YCL, et al. The obesity-associated FTO gene encodes a 2-oxyglutarate-dependent nucleic acid demethylase. Science2007; 318:1469-72.
- Fredriksson R, Hagglund M, Olszewski PK, et al. The obesity gene, FTO, is of ancient origin, upregulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain. Endocrinology2008; 149:2062-71.
- Stratigopoulous G, Padilla S, Leduc CA, et al. Regulation of FTO/FTM gene expression in mice and humans. Am. J. Physiol. Regul. Integr. Comp. Physiol.2008; 294:R1185-96.
Buffer:
FTO Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Immunogen:
FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO.
The immunogen is located within the first 50 amino acids of FTO.
The immunogen is located within the first 50 amino acids of FTO.
NCBI Gene ID #:
79068
NCBI Official Name:
fat mass and obesity associated
NCBI Official Symbol:
FTO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9C0B1
Protein GI Number:
148841515
Purification:
FTO Antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
Swissprot #:
Q9C0B1
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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