RNF168 Antibody
| Code | Size | Price |
|---|
| PSI-5147-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5147-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RNF168 Antibody: hRNF168, E3 ubiquitin-protein ligase RNF168, RING finger protein 168, hRNF168
Application Note:
RNF168 antibody can be used for detection of RNF168 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
RNF168 Antibody: RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.
Background References:
- Doil C, Mailand N, Bekker-Jensen S, et al. RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Cell2009; 136:435-46.
- Pinato S, Scandiuzzi C, Arnaudo N, et al. RNF168, a new RING finger, MIU-containing protein that modifies chromatin by ubiquitination of histones H2A and H2AX. BMC Mol Biol.2009; 10:55.
Buffer:
RNF168 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (89%), Bovine: (82%)
Immunogen:
RNF168 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human RNF168.
The immunogen is located within amino acids 440 - 490 of RNF168.
The immunogen is located within amino acids 440 - 490 of RNF168.
NCBI Gene ID #:
165918
NCBI Official Name:
ring finger protein 168
NCBI Official Symbol:
RNF168
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_689830
Protein GI Number:
31377566
Purification:
RNF168 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q8IYW5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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