beta-Galactosidase Antibody

ProSci
Product Code: PSI-5155
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5155-0.02mg0.02mg£150.00
Quantity:
PSI-5155-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of (A) 5 and (B) 25 ng of b-gal with b-gal antibody at 1 μg/mL.

Western blot analysis of (A) 5 and (B) 25 ng of b-gal with b-gal antibody at 1 μg/mL.

Further Information

Additional Names:
beta-Galactosidase Antibody: Beta-galactosidase, Lactase, Beta-gal
Application Note:
beta-gal antibody can be used for detection of beta-gal by Western blot at 1 μg/mL.
Background:
beta-Galactosidase Antibody: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
Background References:
  • Matthews BW. The structure of E. coli beta-galactosidase. C. R. Biol.2005; 328:549-56.
Buffer:
beta-Galactosidase Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
beta-gal antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of E. coli beta-gal.

The immunogen is located within amino acids 20 - 70 of beta-Galactosidase.
NCBI Gene ID #:
1036228
NCBI Official Name:
lacZ beta-D-galactosidase
NCBI Official Symbol:
lacZ
NCBI Organism:
Escherichia coli CFT073 (strain: CFT073)
Physical State:
Liquid
Protein Accession #:
NP_752394
Protein GI Number:
26246355
Purification:
beta-Galactosidase Antibody is affinity chromatography purified via peptide column.
Research Area:
Other
Swissprot #:
Q8FKG6
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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