Siglec11 Antibody
| Code | Size | Price |
|---|
| PSI-5151-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5151-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Siglec11 Antibody: Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11
Application Note:
Siglec11 antibody can be used for detection of Siglec11 by Western blot at 1 μg/mL. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Siglec11 Antibody: Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10.
Background References:
- Crocker PR and Varki A. Siglecs, sialic acids, and innate immunity. Trends Immunol.2001; 22:337-42.
- Angata T, Kerr SC, Greaves DR, et al. Cloning and characterization of human siglec-11. A recently evolved signaling molecule that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia. J. Biol. Chem.2002; 27:24466-74.
Buffer:
Siglec11 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Siglec11 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11.
The immunogen is located within the last 50 amino acids of Siglec11.
The immunogen is located within the last 50 amino acids of Siglec11.
NCBI Gene ID #:
114132
NCBI Official Name:
sialic acid binding Ig-like lectin 11
NCBI Official Symbol:
SIGLEC11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_443116
Protein GI Number:
205830470
Purification:
Siglec11 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
Q96RL6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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