AP2M1 Antibody
| Code | Size | Price |
|---|
| PSI-5161-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5161-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
AP2M1 Antibody: mu2, AP50, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain
Application Note:
AP2M1 antibody can be used for detection of AP2M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
AP2M1 Antibody: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
Background References:
- Robinson MS. Adaptable adaptors for coated vesicles. Trends Cell Biol.2004; 14:167-74.
- Aguilar RC, Ohno H, Roche KW, et al. Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu J. Biol. Chem.1997; 272:27160-6.
- Liu Q, Feng Y, and Forgac M. Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2. J. Biol. Chem.1994; 269:31592-7.
- Craig HM, Reddy TR, Riggs NL, et al. Interactions of HIV-1 Nef with the m subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif. Virology271:9-17.
Buffer:
AP2M1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
Immunogen:
AP2M1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human AP2M1.
The immunogen is located within amino acids 360 - 410 of AP2M1.
The immunogen is located within amino acids 360 - 410 of AP2M1.
NCBI Gene ID #:
1173
NCBI Official Name:
adaptor-related protein complex 2, mu 1 subunit
NCBI Official Symbol:
AP2M1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004059
Protein GI Number:
14917109
Purification:
AP2M1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q96CW1
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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