ST3gal6 Antibody

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ProSci
Product Code: PSI-5169
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5169-0.02mg0.02mg£150.00
Quantity:
PSI-5169-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of ST3gal6 in HeLa cell lysate with ST3gal6 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of ST3gal6 in HeLa cells with ST3gal6 antibody at 5 μg/mL.
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Western blot analysis of ST3gal6 in HeLa cell lysate with ST3gal6 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of ST3gal6 in HeLa cells with ST3gal6 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
ST3gal6 Antibody: SIAT10, ST3GALVI, SIAT10, Type 2 lactosamine alpha-2, 3-sialyltransferase, ST3Gal VI, ST3GalVI
Application Note:
ST3gal6 antibody can be used for detection of ST3gal6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
ST3gal6 Antibody: Sialyltransferases catalyze the transfer of sialic acid from cytidine 5-prime monophospho-N-acetylneuraminic acid (CMP-NeuAc) to terminal positions of glycoprotein and glycolipid carbohydrate groups. Terminal NeuAc residues are key determinants of carbohydrate structures, such as the sialyl-Lewis X determinants, and are widely distributed in many cell types. However, cancer cells often express more heavily sialylated glycans on their cell surface and this feature sometimes correlates with invasiveness. In contrast, expression of ST3gal6, a member of the sialyltransferase family that sialylates type II lactosamine structures on glycoproteins and glycolipids, was found to be significantly decreased by hypermethylation of the gene in gastrointestinal cancer. At least three isoforms of ST3gal6 are known to exist.
Background References:
  • Dall'Olio F and Chiricolo M. Sialyltransferases in cancer. Glycoconj. J.2001; 18:841-50.
  • Okajima T, Fukumoto S, Miyazaki H, et al. Molecular cloning of a novel alpha2,3-sialyltransferase (ST3Gal VI) that sialylates type II lactosamine structures on glycoproteins and glycolipids. J. Biol. Chem.1999; 274:11479-86.
  • Kawamura YI, Toyota M, Kawashima R, et al. DNA hypermethylation contributes to incomplete synthesis of carbohydrate determinants in gastrointestinal cancer. Gasatroenterology2008; 135:142-51.
Buffer:
ST3gal6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
ST3gal6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ST3gal6.

The immunogen is located within amino acids 230 - 280 of ST3gal6.
NCBI Gene ID #:
10402
NCBI Official Name:
ST3 beta-galactoside alpha-2,3-sialyltransferase 6
NCBI Official Symbol:
ST3GAL6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006091
Protein GI Number:
5174697
Purification:
ST3gal6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
Swissprot #:
Q9Y274
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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