LDL-R Antibody

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ProSci
Product Code: PSI-5163
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5163-0.02mg0.02mg£150.00
Quantity:
PSI-5163-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of LDL-R in human liver tissue lysate with LDL-R antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of LDL-R in human liver tissue with LDL-R antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of LDLR in human liver tissue with LDLR antibody at 20 μg/mL.
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Western blot analysis of LDL-R in human liver tissue lysate with LDL-R antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of LDL-R in human liver tissue with LDL-R antibody at 2.5 μg/mL.
Immunofluorescence of LDLR in human liver tissue with LDLR antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
LDL-R Antibody: FH, FHC, LDLCQ2, Low-density lipoprotein receptor, LDL receptor
Application Note:
LDL-R antibody can be used for detection of LDL-R by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
LDL-R Antibody: The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.
Background References:
  • Li Y, Cam J, and Bu G. Low-density lipoprotein receptor family: endocytosis and signal transduction. Mol. Neurobiol.2001; 23:53-67.
  • Austin MA, Hutter CM, Zimmern RL, et al. Familial hypercholesterolemia and coronary heart disease: a HuGE association review. Am. J. Epidemiol.2004; 160:421-9.
  • Helle F and Dubuisson J. Hepatitis C virus entry into host cells. Cell Mol. Life Sci.2008; 65:100-12.
Buffer:
LDL-R Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (89%), Rabbit: (80%)
Immunogen:
LDL-R antibody was raised against an 18 amino acid synthetic peptide near the center of human LDL-R.

The immunogen is located within amino acids 490 - 540 of LDL-R.
NCBI Gene ID #:
3949
NCBI Official Name:
low density lipoprotein receptor
NCBI Official Symbol:
LDLR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000518
Protein GI Number:
4504975
Purification:
LDL-R Antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
Swissprot #:
P01130
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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