CLDN1 Antibody

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ProSci
Product Code: PSI-5187
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5187-0.02mg0.02mg£150.00
Quantity:
PSI-5187-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (1 μg/mL), CLDN1, 24-027 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human HepG2 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (1 μg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (1 μg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Immunofluorescence Validation of CLDN1 in HepG2 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with 5187 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (1 μg/mL), CLDN1, 24-027 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human HepG2 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (1 μg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLDN1, 5187 (1 μg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of CLDN1 in HepG2 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with 5187 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
CLDN1 Antibody: CLD1, SEMP1, ILVASC, CLD1, UNQ481/PRO944, Claudin-1, Senescence-associated epithelial membrane protein
Application Note:
WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CLDN1 Antibody: Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.
Background References:
  • Tepass. Nat. Cell Biol.2003; 5:595-7.
  • Kinugasa et al. Anticancer Res.2007; 27:3729-34.
  • Kinugasa et al. Anticancer Res.2007; 27:3729-34.Mod. Pathol.2007; 20:947-954.
  • Morohashi et al. Int. J. Mol. Med.2007; 20:139-43.
Buffer:
CLDN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Mouse (95%)
Immunogen:
Anti-CLDN1 antibody (5187) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human CLDN1.

The immunogen is located within the last 50 amino acids of CLDN1.
ISOFORMS:
Human CLDN1 has one isoform (211aa, 23kD). Mouse CLDN1 has one isoform (211aa, 23kD) and Rat CLDN1 also has one isoform (211aa, 23kD). 5187 can detect human, mouse and rat.
NCBI Gene ID #:
9076
NCBI Official Name:
claudin 1
NCBI Official Symbol:
CLDN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 23kD

Observed: 23 kD
Protein Accession #:
CAG33419
Protein GI Number:
48146393
Purification:
CLDN1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
Swissprot #:
O95832
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar CLDN1 expression profile in human cell lines detected by two independent anti-CLDN1 antibodies that recognize different epitopes, 5187 against C-terminus domain and 24-027 against recombinant fusion protein.  CLDN1 proteins are detected in some tested cell lines at different expression levels by the two independent antibodies. 

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