MACC1 Antibody
| Code | Size | Price |
|---|
| PSI-5197-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5197-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
MACC1 Antibody: 7A5, SH3BP4L, Metastasis-associated in colon cancer protein 1, SH3 domain-containing protein 7a5
Application Note:
MACC1 antibody can be used for detection of MACC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
MACC1 Antibody: Metastasis associated in colon cancer 1 (MACC1) is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. MACC1 protein consists of four domains: ZU5, SH3, and two C-terminal death domains (DD). Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment.
Background References:
- Stein U, Walther W, Arlt F, et al. MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis. Nat. Med.2009; 15:59-67.
- Boardman LA. Overexpression of MACC1 leads to downstream activation of HGF/MET and potentiates metastasis and recurrence of colorectal cancer. Genome Med.2009; 1:36.
- Stein U, Smith J, Walther W, et al. MACC1 controls Met: what a difference an Sp1 site makes. Cell Cycle2009; 8:2467-9.
- Arlt F and Stein U. Colon cancer metastasis: MACC1 and Met as metastatic pacemakers. Int. J. Biochem. Cell Biol.2009; epub.
Buffer:
MACC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1.
The immunogen is located within amino acids 430 - 480 of MACC1.
The immunogen is located within amino acids 430 - 480 of MACC1.
NCBI Gene ID #:
346389
NCBI Official Name:
metastasis associated in colon cancer 1
NCBI Official Symbol:
MACC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 94 kDa
Observed: 90 kDa
Observed: 90 kDa
Protein Accession #:
NP_877439
Protein GI Number:
157502191
Purification:
MACC1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
At least two isoforms of MACC1 are known to exist.
Swissprot #:
Q6ZN28
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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