RGS21 Antibody
| Code | Size | Price |
|---|
| PSI-5209-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5209-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RGS21 Antibody: Regulator of G-protein signaling 21, RGS21
Application Note:
RGS21 antibody can be used for detection of RGS21 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RGS21 Antibody: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.
Background References:
- De Vries L, Mousli M, Wurmser A, et al. GAIP, a protein that specifically interacts with the G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA1995; 92:11916-20.
- Berman DM, Wilkie TM, and Gilman AG. GAIP and RGS4 are GTP-ase activating proteins for the Gi subfamily of G protein alpha subunits. Cell1996; 86:445-52.
- von Bucholtz L, Elischer A, Tareilus E, et al. RGS21 is a novel regulator of G protein signalling selectively expressed in subpopulations of taste bud cells. Eur. J. Neurosci.2004; 19:1535-44.
- Li X, Chen L, Ji C, et al. Isolation and expression pattern of RGS21 gene, a novel RGS member. Acta Biochim. Pol.2005; 52:943-6.
Buffer:
RGS21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RGS21 antibody was raised against a 16 amino acid synthetic peptide near the center of human RGS21.
The immunogen is located within amino acids 40 - 90 of RGS21.
The immunogen is located within amino acids 40 - 90 of RGS21.
NCBI Gene ID #:
431704
NCBI Official Name:
regulator of G-protein signaling 21
NCBI Official Symbol:
RGS21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001034241
Protein GI Number:
85540441
Purification:
RGS21 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q2M5E4
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier | RGS21 Peptide | PSI-5209P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|