Fibulin 3 Antibody
| Code | Size | Price |
|---|
| PSI-5213-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5213-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Fibulin 3 Antibody: DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, FBLN3, FIBL-3, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5
Application Note:
Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Fibulin 3 Antibody: Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target.
Background References:
- Kobayashi N, Kostka G, Garbe JH, et al. A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization. J. Biol. Chem. 2007; 282:11805-16.
- Marmorstein LY, Munier FL, Arsenijevic Y, et al. Aberrant accumulation of EFEMP1 underlies drusen formation in Malattia Leventinese and age-related macular degeneration. Proc. Natl. Acad. Sci. USA 2002; 99:13067-72.
- McLaughlin PJ, Bakall B, Choi J, et al. Lack of fibulin-3 causes early aging and herniation, but not macular degeneration in mice. Hum. Mol. Genet. 2007; 16:3059-70.
- Seeliger H, Camaj P, Ischenko I, et al. EFEMP1 expression promotes in vivo tumor growth in human pancreatic adenocarcinoma. Mol. Cancer Res. 2009; 7:189-98.
Buffer:
Fibulin 3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3.
The immunogen is located within amino acids 410 - 460 of Fibulin 3.
The immunogen is located within amino acids 410 - 460 of Fibulin 3.
NCBI Gene ID #:
2202
NCBI Official Name:
EGF-containing fibulin-like extracellular matrix protein 1
NCBI Official Symbol:
EFEMP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 52 kDa
Observed: 47 kDa
Observed: 47 kDa
Protein Accession #:
NP_001034437
Protein GI Number:
9665262
Purification:
Fibulin 3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
Swissprot #:
Q12805
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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